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Macrogen offers DNA sequencing for difficult templates with high GC or GT, unusual secondary structure or hairpin structures. With over 20 years of experiences, we have optimized our protocol to yield successful sequencing data for these difficult templates.

Macrogen provides premium protocol for difficult template sequencing.
- High GC or GT
- Unusual secondary structure
- Repetitive sequence
- siRNA with hairpin structure
- Homopolymeric tracts (PolyA, PolyG)
 
Macrogen offers sequencing service for 16S/18S rRNA gene from your bacterial or fungal colonies rapidly and effectively. We provide all-inclusive service performing DNA extraction, PCR, sequencing and assembly.



Performs all processes including nucleic acid extraction, PCR, sequencing, assembly using bacterial cells.
Deliver results within 7 business days from date of sample arrival
 
As shown in the figure below, living organisms can be classified into 3 primary lineages using the base sequence analysis of 16S rRNA: Domain Eucarya, Domain Bacteria, and Domain Archaea.



Macrogen offers primer walking service to confirm the sequence integrity of your clone or PCR products. We can perform end sequencing with the primers that you provide or use the reference sequence, if applicable, and design internal primers to fill in the gap.
1Kb single contig : 5~6 days.
  (Time consuming would vary based on the following reasons; primer synthesis and sequencing
    failure)
Phred score of 20 or higher quality contig is requirerd.
Time can be saved when primers are synthesized bidirectionally.
  (refer to the figure as follows)
 
 
Starting with the end sequencing product by using the primer provided or designed,
  internal primer will be designed and processed.
 
Using a new primer (but with the same template), internal primer continues reading at
  the appropriate location.
 
Each walking requires approximately 5~6 days; enabling extension of 500bp~600bp per direction.
 

Number of primers and sequence used for primer walking

Abi file used for primer walking
Contig sequence and contig quality