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Samples and primers can be submitted in 1.5 ml tubes, strip tubes or 96 well PCR plates.

As for the DNA samples in water or TE, bacterial cells in agar-stab, or agar plate culture, temperature control is not necessary. Samples are stable for a few days at room temperature.

a) Sample for individual tube :
- General glycerol stock/ Agar-stab/Agar-plate culture at room temperature.
- 1.5mL Microcentrifuge Tube is recommended in solution (Nuclease-free TE or distilled water) at room temperature.
- Free re-sequencing is included

b) 96 Well Plate :
- 8 strip cap is recommended in solution (Nuclease-free TE or distilled water) at room temperature.
- Re-sequencing is additionally charged.

*-U-bottom and Flat-bottom plate are not accepted.

** In case of Multi primers for one plate **
Only two custom primers (or two universal primers) in tubes could be acceptable for applying this Plate sequencing.

* Note:
- For three or more primers, another paired primer plate with your sample….reactions.
- The well to well variation of the concentration of your samples is very important to get
   better results, please keep it as constant as possible.



- Please prepare samples to avoid any well-to-well concentration difference or size difference for
  quality results.

c) Primer :
- Primer : 2-5 pmol/ul(uM) in 20µl based on 5 samples
- Primer Tm is suitable for service : 55°C ~ 60°C
- Commonly used Universal primers are available for free of charge (ex. M13F, T7, T3)
- Design and synthesize primers if you request. (US $0.35/base)

d) To apply Plate sequencing :
- 1 primer/1plate service :

- Multi primers/ 1plate service :
  Only two custom primers (or two universal primers) in tubes could be acceptable for applying this Plate
sequencing.

Note : For three or more primers, another paired primer plate with your sample plate should be provided. If not, your order will be regarded as Normal sequencing reactions.


- Sealing of 96 Well Plates
- Strip caps are recommended to seal 96-well plates.
- Carefully seal the 96 well plates with strip caps (8-strips or 12-strips) or polypropylene films
   (relatively thick and malleable).
- Aluminum sealing or polyester films are not recommended.

Place samples into strip-capped well plate as shown below.
To avoid potential damage, please use out-skirted well plate.


Seal tightly to avoid sample evaporation or contamination during transit.

Unsatisfactory results due to improper sample preparation or shipping by customer will be charged for re-sequencing.